The cave Myotis varies in appearance depending on region, with individuals in the eastern portion of the range having paler brown fur and individuals in the west appearing darker brown. The face, ears, and wing membranes are a dark, dusky color. Prey includes moths, beetles, weevils, and other insects. The cave Myotis prefers forested habitat across a range of elevations. This species roosts in caves, tunnels, buildings, and abandoned mines. Some cave Myotis have been observed migrating south for winter, while others hibernate near their summer habitat. Disturbance and encroaching vegetation that impedes access to cave roosts pose a threat to this species.